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KMID : 1134820160450060911
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Journal of the Korean Society of Food Science and Nutrition
2016 Volume.45 No. 6 p.911 ~ p.917
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Inhibition of Cell Proliferation and Induction of Apoptosis by Ethanolic Extract of Lespedeza cuneata G. Don in Human Colorectal Cancer HT-29 cells
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Zhao Qian
Kim Yeah-Un
Han In-Hwa
Yun Jung-Mi
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Abstract
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Lespedeza cuneata G. Don is an edible perennial herb used in traditional Korean medicine. We investigated the anti-proliferative properties and mechanism of L. cuneata extract. The ethanolic extract of L. cuneata dose- and time-dependently inhibited human colorectal cancer cell proliferation. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to test the effect of the extract on proliferation of HT-29 colorectal cancer cells. The extract inhibited HT-29 cell proliferation with an IC50 value of 554.26±8.81 μg/mL. L. cuneata extract suppressed production of pro-inflammatory cytokines interleukin-6 and tumor necrosis factor-α. Apoptosis was evaluated by analysis of DNA fragmentation, poly(ADP-ribose) polymerase cleavage, caspase-3 activity, and protein expression of pro-apoptotic (Bax) and anti-apoptotic (Bcl-2). Our results demonstrated that the extract induced DNA fragmentation and characteristic morphological changes associated with apoptosis in HT-29 colorectal cancer cells. The extract also time- and dose-dependently up-regulated expression of the Bax and down-regulated expression of the Bcl-2. Furthermore, the extract dose- and time-dependently enhanced caspase-3 activity. Our findings provide evidence that L. cuneata extract may mediate its anti-proliferative effect via modulation of apoptosis.
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KEYWORD
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Lespedeza cuneata G. Don, HT-29 colorectal cancer cells, apoptosis, antitumor activity, anti-inflammation
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